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Southeast Ecological Science Center

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U.S. Geological Survey
Florida Integrated Science Center

Amphibian Field Research
Preventing the Spread of Disease Among Populations


A Word of Caution: Biosecurity

Concern about disease and toxic contamination as causes of amphibian declines has increased considerably in recent years. A corollary of this concern is the need for field workers to avoid becoming vectors for transmitting disease organisms or toxic chemicals among study sites. The Declining Amphibian Populations Task Force (DAPTF) has developed a standard protocol for use by anyone conducting fieldwork at amphibian breeding sites or in other aquatic habitats. These procedures will be used for all routine surveys, but more stringent measures will be necessary in areas with known disease problems.

BIOSECURITY PROTOCOL

Protective Wear & Equipment
Disinfecting & Sanitizing Methods

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Non-permeable boots or waders

rinse in bleach solution immediately after leaving each study site

Vinyl gloves

dispose of gloves after each handling incident

Nets

rinse in bleach solution immediately after leaving each study site

Plastic bags (for holding specimens) * One bag per specimen

dispose of after each use

Needles & syringes (for blood extraction)

dispose of after each use

Scalpel blades, PIT tag cannula, Forceps, etc.

immerse in sterilizing solution

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SOLUTION FORMULAS

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Bleach

1% sodium hypochlorite

sanitizing solution (for instruments)

70% methanol for 30 minutes, then flamed; or, 1% glutaraldehyde for 15 minutes; or, boiling water for 10 minutes

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ADDITIONAL PRECAUTIONS

 1. Avoid contact between used and unused protective wear and equipment.
 2. House specimens separately.
 3. Avoid contact between gloved hands and face, especially the area of the nose.
 4. DO NOT urinate in or near ponds and streams.
 5. Wash hands thoroughly with soap and water, or use a sanitary wipe, after urinating.
 6. Wash hands thoroughly with soap and water, or use a sanitary wipe, after handling specimens known
     or suspected of being diseased or contaminated.
 7. Wash hands thoroughly with soap and water, or use a sanitary wipe, after leaving each site.

 

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Page Last Modified: Friday, 14-Dec-2012 12:56:53 EST